Differentiated Epithelial- and Mesenchymal-Like Phenotypes in Subcutaneous Mouse Xenografts Using Diffusion Weighted-Magnetic Resonance Imaging
نویسندگان
چکیده
Epithelial-mesenchymal transition (EMT) is important for tumor metastasis. Detection of EMT protein expression and observation of morphological changes are commonly used to identify EMT. Diffusion-weighted magnetic resonance imaging (DW-MRI) and measuring apparent diffusion coefficient (ADC) values are noninvasive techniques for characterizing tumor microenvironments. We investigated the difference in ADC values between epithelial- and mesenchymal-like subcutaneous mouse xenografted tumors using DW-MRI. Epithelial-like MM189 PB-Klf4 and BL322 PB-Klf4 cells were generated from tumor suppressive Kruppel-like factor 4 (Klf4)-expressing mesenchymal-like MM189 and BL322 cells. The ADC values of xenografted tumors from epithelial-like MM189 PB-Klf4 and BL322 PB-Klf4 were significantly lower than those from their mesenchymal-like counterparts (p < 0.05 and p < 0.01, respectively). Our results suggested that DW-MRI is a potential tool for observing mesenchymal- or epithelial-like characteristics of subcutaneous xenografted tumors.
منابع مشابه
MR diffusion-weighted imaging-based subcutaneous tumour volumetry in a xenografted nude mouse model using 3D Slicer: an accurate and repeatable method
Accurate and repeatable measurement of the gross tumour volume(GTV) of subcutaneous xenografts is crucial in the evaluation of anti-tumour therapy. Formula and image-based manual segmentation methods are commonly used for GTV measurement but are hindered by low accuracy and reproducibility. 3D Slicer is open-source software that provides semiautomatic segmentation for GTV measurements. In our s...
متن کاملDynamic Contrast Magnetic Resonance Imaging (DCE-MRI) and Diffusion Weighted MR Imaging (DWI) for Differentiation between Benign and Malignant Salivary Gland Tumors
Background: Salivary gland tumors form nearly 3% of head and neck tumors. Due to their large histological variety and vicinity to facial nerves, pre-operative diagnosis and differentiation of benign and malignant parotid tumors are a major challenge for radiologists. Objective: The majority of these tumors are benign; however, sometimes they tend to transform into a malignant form. Functional M...
متن کاملClinical Significance of Diffusion Weighted Magnetic Resonance Imaging on Treatment Efficacy in MS Patients With Acute Attacks
Background: Despite the existence of various imaging methods, the accurate diagnosis of many neurodegenerative diseases is still controversial. The use of advanced imaging techniques, such as the diffusion weighted imaging can be help the early detection of MS and evaluation of the treatment efficacy in these patients. Materials and method: 24 MS patients with acute attack and thirty healthy s...
متن کاملMagnetic resonance imaging of feline eye
The purpose of this study was to investigate magnetic resonance imaging (MRI) of the normal feline eyeand optic nerves using T1-weighted and T2-weighted images. A total of 6 healthy female domestic short haircats age 2-2.5 years and weighing 3.2 ± 0.4 kg were selected. Magnetic resonance imaging data werecollected using GEMSOW (Philips) at a magnetic field strength of 1.5 T. Dorsal, sagittal, a...
متن کاملDynamic contrast enhanced magnetic resonance imaging of an orthotopic pancreatic cancer mouse model.
Dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) has been limitedly used for orthotopic pancreatic tumor xenografts due to severe respiratory motion artifact in the abdominal area. Orthotopic tumor models offer advantages over subcutaneous ones, because those can reflect the primary tumor microenvironment affecting blood supply, neovascularization, and tumor cell invasion. We have...
متن کامل